skip to content

Darwin Correspondence Project

To Nature   15 August [1877]1

The Contractile Filaments of the Teasel

The observations of my son Francis on the contractile filaments protruded from the glands of Dipsacus, offer so new and remarkable a fact in the physiology of plants, that any confirmation of them is valuable.2 I hope therefore that you will publish the appended letter from Prof. Cohn, of Breslau, whom every one will allow to be one of the highest authorities in Europe on such a subject. Prof. Cohn’s remarks were not intended for publication, but he has kindly allowed me to lay them before your readers.3

Extract from Prof. Cohn’s Letter:

“Immediately after the receipt of your very kind letter of July 26 I went to fetch Dipsacus, several species of which grow in our Botanic Garden; and proceeding after your recommendations, I put transverse sections of the cup-like bases of young leaves, or the epidermis of these parts carefully removed from the green parenchyma, into distilled water.4 I thus had the pleasure of witnessing with my own eyes this most curious discovery. First I ascertained the anatomical structure of the pear-like glands which are rather elegant and remarkable. From the basal cell rises the stalk-cell, in the second story there are two cells, in the third four, and in the uppermost series eight cuneiform cells converging to the centre. But you may conceive how much I was surprised by seeing the filiform protuberances issuing from the apex of the glands; it was quite a perplexing spectacle. The filaments are, in their refrangibility, very like the pseudopodia of some Rhizopods (e.g., Arcella or Difflugia).5 I followed their changes for some time, and remarked quite definitely, as I find described in the paper of Mr. Francis Darwin how the protuberances slowly lengthen out, crook themselves hooklike or winding, and get knobbed either at the summit or midway; I saw the knobs or beads glide down the thread, and at last be sucked into a globular mass adhering to the gland. I saw the protuberances always rise between the septa of two or more adjoining cells, but nearly as frequently between the lateral septa as on the apical centre. Generally there were many protuberances on the same gland, pressed forward out of different spots; sometimes I saw two diverging branches proceed from the same point like a pair of compasses, each behaving independently in its changes. But the most curious appearance in these protuberances was a constant waving undulation along their extension, sometimes slower and perceptible with difficulty, sometimes vigorous and quicker, but never ceasing; more delicate filaments appeared to me very like Vibrio, or the vibratory flagella of some Infusoria.6 Not finding a special description of the waving movements of the filaments in your son’s paper, I asked some of my pupils if they saw anything remarkable in the filaments, without indicating what, but they all took the same impression as myself. The only facts I have not yet been able to witness of your son’s discoveries are Figs. 6, 14, 15, and the moniliform contraction; nor have I yet found time to apply chemical reagents, of which your son has made such good use.7

Of course I am not able, after two days’ inspection, to form a definite judgment about the true nature of the filiform protuberances. Putting aside the hypothesis of a parasitic Rhizopod, there are two probabilities which still balance in my mind, as clearly stated by your son. (1) The protuberances are secretions of some colloidal matter, absorbing water, but insoluble in it; the movements are physical (not vital ones), the elongation of the filaments depending on the imbibition, their contraction on the withdrawal of water by different reagents. There are such substances, e.g., myeline, which shows rather similar changes in water.8 Please also to repeat the experiments I performed at the meeting of the British Association last year. Into a cylindrical glass containing soluble silicate of alkali (Wasserglas), diluted with half its amount of water, put a small piece of crystallised chloride of iron; from the fragment there rises a hollow reddish tube growing upwards and moving very quickly, like an Enteromorpha.9 But if you put into the diluted silicate some protochloride of iron (the latter is usually in the form of a powder, but may easily be brought by gentle pressure of the fingers into crumb-like masses), then from the lumps there arise innumerable filaments, very delicate and transparent, very like the glass threads of Hyalonema, which rise in fascicules vertically till they reach the surface of the fluid.10

But I cannot deny that the general impression produced by Dipsacus does not contradict the hypothesis that the changes of the filaments are the vital phenomena of protoplasmic pseudopodia.

A French biologist (whose name I cannot just now remember) has proved many years ago (I think in a early number of the Bull. de la Soc. Bot. de France) that the water in the cups of Dipsacus is not a simple collection of rain in a gutter, but a secretion of the leaf bases.11 If this be truly the case, it is quite probable that the glands may have a special adaptation for this purpose. Indeed, I should not hesitate to agree with the vital theory, if there were any analogy known in plants. But further study of the phenomenon and the repetition of the chemical reactions which your son has already indicated, will, I hope, in a short time enable me to form a more decided judgment in this perplexing dilemma.

In the meantime I am happy to congratulate Mr. Francis Darwin and yourself on account of the extraordinary discovery he has made, and the truly scientific paper in which he has elaborated it, and which has added a series of quite unexpected facts to the physiology of plants.”

In a subsequent letter, Prof. Cohn describes what appear to him as thinned points or pores in the cell wall of the glands from which the filaments seem to be protruded.12 He also mentions the very curious fact which he has discovered, that by adding iodine to the detached epidermis of the leaf cups of Dipsacus the whole fluid contents of the epidermis cells turn blue like diluted starch paste, although no starch grains are met with in any epidermis cell except in the stomata. He adds that the basal cell of the gland becomes blue, while the rest of it and the excreted globules are stained yellow.

I may add that I have heard from Prof. Hoffmann, of Giessen, that he formerly observed contractile filament of a somewhat similar nature on the annulus of Agaricus muscarius. He has described them in the Botanische Zeitung, 1853, and figured them, ibid., 1859, tab. xi. Fig. 17.13

Charles Darwin

Down, Beckenham, August 15

Footnotes

The year is established by the publication date of the letter in Nature.
Francis Darwin had discovered protoplasmic filaments protruding from the glandular hairs of leaves of common or fuller’s teasel (Dipsacus sylvestris, a synonym of D. fullonum; see Correspondence vol. 24, letter from Francis Darwin, [28 May 1876]). In the published paper in the Quarterly Journal of Microscopical Science (F. Darwin 1877b, p. 272), Francis hypothesised that the protrusion of the filaments in some way corresponded to the process of aggregation seen in insectivorous plants like those of the genus Drosera (sundews). CD had been upset when the Council of the Royal Society of London decided not to publish the full paper, only an abstract (F. Darwin 1877a; see letter to G. J. Romanes, 23 May 1877).
See letter to F. J. Cohn, 8 August 1877, and letter from F. J. Cohn, [10?] August 1877. The extract has minor corrections to the English of the original letter from Cohn of 5 August 1877; Cohn had agreed that CD make the changes for publication (see letter from F. J. Cohn, [10?] August 1877).
In his letter to Cohn of 26 July 1877, CD had advised Cohn on the best method of preparing leaves in order to see the filaments described in F. Darwin 1877b.
Rhizopoda is a wide group of protozoan amoeboid organisms characterised by the possession of pseudopodia. Arcella and Difflugia are genera of testate amoebae within the Rhizopoda; they use pseudopods, which extrude from their shells, to move and to capture prey.
Cohn had published the first sytematic classification of bacteria in 1872 (Cohn 1872); Vibrio refers to Cohn’s genus of rod-like bacterial organisms characterised by the vibratory motion of the filaments (see Cohn 1872, pp. 178–9).
For the chemical reagents that Francis used, see F. Darwin 1877b, pp. 250–6. For the figures Cohn refers to, see ibid., plate xix.
Myeline (now myelin) is an organic compound that constitutes the insulating layer around nerve fibres in animals or various tubular lamellar structures in both animal and plant cells. Observers had noted that when water was added to dry myeline, flexible tubular structures extruded from the margins (see, for example, Edmund Montgomery 1866, p. 317).
Cohn had presented a paper, ‘Experiments on the formation and growth of artificial silica cells’, to the botany and zoology section of the meeting of the British Association at Glasgow in September 1876; only the title of the paper appeared in the Report of the 46th meeting of the British Association for the Advancement of Science (1876), Transactions of the sections, p. 146. Wasserglas or soluble glass is a popular name for any of the soluble alkaline silicates such as sodium silicate. The addition of a metal salt such as ferric chloride (crystallised chloride of iron) results in a salt metathesis (double displacement) reaction and the upward growth of the precipitate is caused by the differential pressure of the solvent in the container; the surface of the precipitate behaves like a semipermeable membrane. Enteromorpha (a synonym of Ulva) is the genus of green nori (sea lettuce).
Protochloride of iron is generally referred to now as ferrous chloride (FeCl2). Hyalonema is a genus of sponge, sometimes referred to as glass rope sponge.
Cohn refers to an article by Charles Royer, ‘Note sur l’eau des feuilles du Dipsacus silvestris Mill.’, that appeared in 1863 in the Bulletin de la Société botanique de France; Royer concluded that most of the fluid in the cups of Dipsacus sylvestris was the result of secretion rather than accumulation of dew or rainwater (Royer 1863, p. 747).
No letter from Hermann Hoffmann mentioning his articles ‘Ueber contractile Gebilde bei Blätterschwämmen’ (On contractile bodies in gilled mushrooms; Hoffmann 1853) and ‘Ueber Pilzkeimungen’ (On mushroom germination; Hoffmann 1859) has been found. Agaricus muscarius is a synonym of Amanita muscaria, the fly agaric mushroom.

Bibliography

Correspondence: The correspondence of Charles Darwin. Edited by Frederick Burkhardt et al. 27 vols to date. Cambridge: Cambridge University Press. 1985–.

Hoffmann, Hermann. 1853. Ueber contractile Gebilde bei Blätterschwämmen. Botanische Zeitung 11: 857–66.

Hoffmann, Hermann. 1859. Ueber Pilzkeimungen. Botanische Zeitung 17: 209–14, 217–19.

Montgomery, Edmund. 1866. On the formation of cells in animal bodies. [Read 20 December 1866.] Proceedings of the Royal Society of London 15 (1866–7): 314–18.

Royer, Charles. 1863. Note sur l’eau des feuilles du Dipsacus silvestris Mill. Bulletin de la Société botanique de France 10: 746–8.

Summary

CD forwards letter from F. J. Cohn [11093] that provides confirmation of observations by Francis Darwin on the contractile filaments protruded from the glands of Dipsacus.

Letter details

Letter no.
DCP-LETT-11108
From
Charles Robert Darwin
To
Nature
Sent from
Down
Source of text
Nature, 23 August 1877, p. 339

Please cite as

Darwin Correspondence Project, “Letter no. 11108,” accessed on 3 August 2021, https://www.darwinproject.ac.uk/letter/DCP-LETT-11108.xml

letter